Journal: European Radiology Experimental

Article Title: [Fe-EOB-tCDTA] generates strong contrast in the blood but not in the liver, despite inhibiting cellular [Gd-EOB-DTPA] 2- uptake and partial liver excretion

doi: 10.1186/s41747-026-00731-0

Figure Lengend Snippet: Toxicity of [Fe-EOB-tCDTA] and [Gd-EOB-DTPA] 2 - in BRL-3A cells. The cells were exposed to different concentrations of contrast agents (0, 1, 2.5, 5, 10, and 20 mM) for 2 h. Viability was determined using the MTT assay after incubation (three replicates, n = 9, mean ± standard error). Statistical analyses were performed using multiple unpaired t -tests. No significant differences were observed between the various concentration groups

Article Snippet: BRL-3A cells (ATCC, Manassas, VA, USA) were grown under the following conditions: DMEM/F12 medium containing GlutaMAX, 10% fetal bovine serum, 1,000 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific) at 37 °C with 5% CO2.

Techniques: MTT Assay, Incubation, Concentration Assay

Journal: European Radiology Experimental

Article Title: [Fe-EOB-tCDTA] generates strong contrast in the blood but not in the liver, despite inhibiting cellular [Gd-EOB-DTPA] 2- uptake and partial liver excretion

doi: 10.1186/s41747-026-00731-0

Figure Lengend Snippet: Laser ablation–inductively coupled plasma–mass spectrometry of [Fe-EOB- t CDTA] and [Gd-EOB-DTPA] 2 - in BRL-3A cells. a 56 Fe mass spectrometry signal of endogenous iron and of [Fe-EOB- t CDTA]; b 158 Gd mass spectrometry signal of [Gd-EOB-DTPA] -2 . The BRL-3A cells were treated with [Gd-EOB-DTPA] 2 - only (1 mM) or with [Gd-EOB-DTPA] 2 - and [Fe-EOB- t CDTA] at different molar ratios (1:0.5, 1:1, 1:2.5, 1:5, 1:10, and 1:20). Blank: No metal complexes.

Article Snippet: BRL-3A cells (ATCC, Manassas, VA, USA) were grown under the following conditions: DMEM/F12 medium containing GlutaMAX, 10% fetal bovine serum, 1,000 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific) at 37 °C with 5% CO2.

Techniques: Clinical Proteomics, Mass Spectrometry